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Exact(7)
Therefore, the development of species-specific primers would be necessary for obtaining SSR markers for functionally important genes.
To develop SSR markers for functionally important genes, primer sets were designed based on the sequences of nine-spined sticklebacks using WebSat [ 82].
Secondly, we investigated the potential utility of available sequenced fish genomes to develop SSR markers for functionally important genes in nine-spined sticklebacks.
Another possibility is that cancer-driving mutations are in the regulatory parts of the genes, and the coding SNPs in the present study can be considered markers for functionally active regulatory sites.
To develop SSR markers for functionally important genes in nine-spined sticklebacks, SSR locations were surveyed in or around 67 target genes based on the three-spined stickleback genome and these regions were sequenced with primers designed from conserved sequences in sequenced fish genomes.
We investigated the potential utility of available sequenced fish genomes to develop microsatellite (cf. simple sequence repeat, SSR) markers for functionally important genes in nine-spined sticklebacks (Pungitius pungitius), as well as cross-species transferability of SSR primers from three-spined (Gasterosteus aculeatus) to nine-spined sticklebacks.
Similar(53)
We used the high capacity receptor-mediated endocytosis of soluble waste molecules as a marker for functionally intact cells in the cultures.
The gene Urocortin 3 (Ucn3) is a marker for functionally mature β cells, cells capable of glucose stimulated insulin secretion (Blum et al., 2012).
Prominent expression of NPY by subplate neurons from 16 postconceptional weeks (PCW) in the human has been previously described (Delalle et al. 1997) and is considered a marker for functionally mature neurons in the subplate (Chun et al. 1987).
While not selected relative to specific disease or expression outcome, fitSNPs represent a set of markers enriched for functionally relevant variants (e.g. non-synonymous coding region polymorphisms) from across the genome.
The SSR markers developed for functionally important genes should be useful to identify genes responsible for phenotypic variation and adaptive divergence in nine-spined sticklebacks, as well as for constructing comparative gene maps of nine-spined and three-spined sticklebacks.
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