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At the seedling stage, recombinants between two DNA markers encompassing the HWC2 locus were selected and transplanted individually in plastic pots.
Ninety-three markencompassingsing the whole yeast nuclear genome were designed to genotype the 94 gametes, to evaluate the potential ascertainment bias (Figure 2A; Table S1).
To confirm that the deletion extended to the centromere, we further performed LOH-associated SNP loci analysis on five selected 1p/19q oligodendroglioma cases using a panel of 39 single copy SNP (single-nucleotide polymorphisms) markers encompassing the NOTCH2 locus.
Non-significant markers encompassing the region were respectively at 119.07 (F3SDQUK02B73C1_105) and 123.28 Mb (FSIEL0M01A1W48_163).
There are 1287 MSY specific STS markers encompassing the human Y chromosome with an average spacing of 14 Kb spanning over 1698 loci [ 17].
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In the present study, a sequence-tagged site (STS) marker C11112 and a cleaved amplified polymorphic sequence (CAPS) marker C1016 (Table 3, Fig. 1a, b) were used for selecting recombinants around the HWC2 locus in 2003 because the two markers encompass the two RFLP markers and show polymorphism between the two cultivars.
In L. perenne, the genetic distance spanned by the DArT markers encompassed the majority of the regions that could be described in terms of comparative genomic relationships with rice, Brachypodium distachyon, and Sorghum bicolor.
This 1.2 Mb segment, mapped in the central region of the 8.1 haplotype between the BAT3 and C3-2-11 C3-2-11 C3-2-11asses the Class III and proximarkersss I loci and constitutencompassesal causathee loClasstermed MYAS1 [ 37].
The non-luminal group, showing lower levels of luminal markers and high levels of basal markers, encompassed the previously clustered normal breast tissue, HB4a and PMC42, but also BT20 (a known basal cell line, confirmed by cytokeratin 5/6 immunostaining; data not shown).
(PDF 23911 kb) Additional file 5: The SLAF markers encompassed in the major sex associated locus as revealed by QTL analysis.
The manifestation of the basic defect was associated with markers rs11807298 rs6684219, encompassing the CLCA4 promoter (Praw=0.0013; Praw=0.00137).
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