The NCI recommended five markers comprising the National Cancer Institute Consensus Panel (NCI-CP) for the detection of MSI, i.e. markers for the mononucleotide repeats BAT25 and BAT26 and the dinucleotide repeats D2S123, D5S346 and D17S250 [ 48].
To address these questions, we used nuclear microsatellite markers, as well as mtDNA markers, comprising the next logical step to the previous study of Bilgin et al. [ 12], which had used only mtDNA data to mainly evaluate the taxonomic status of M. schreibersii in this region.
The combined dataset of the three genetic markers comprised the sequences of three outgroup species, 38 Burkholderia species, five nodulating endophytes and the endophytes of 28 gousiekte-inducing plants.
The variables (analytical markers) comprised the amino acid composition, carbonyl index, crystalline index, molar mass from SEC, acidity, Young's modulus, elongation at break and tenacity at break (see Electronic Supplementary Material).
BLAST searches were used to determine putative locations for the 64-bp sequences of the 2235 GBS markers comprising our final linkage map in the Astyanax genomic and transcriptomic data sets.
The markers comprising each haplotype block are listed in Table S2 and association data for haplotypes with a frequency greater than 0.1% are reported in Table S3.
These clusters and the individual markers comprising them were able to demonstrate relationships with affective symptomatology.
Moreover, the number of markers comprising bins of common frequency (6,894,397 markers with MAF > 0.05) is much lower than the number of markers comprising bins of low frequency (23,178,341 markers with MAF < 0.05), which usually have low imputation quality.
The 24 consensus LGs covered a distance of 1,320 cM, with a length ranging from 78 to 145 cM, and the distance between adjacent markers (comprising all types of markers without accessory markers) averaging 4 cM.
Promoter methylation was evaluated for the CIMP panel of markers comprising CACNA1G, IGF2, NEUROG1, RUNX3 and SOCS1, where PMR (percentage methylated reference) values were derived using the ALU normalisation control reaction (Weisenberger et al, 2006).
The combined genetic linkage maps contain 2078 markers comprising 1793 DArT, 258 AFLP, and 25 SSR DNA markers, plus the two markers that co-segregate with the biological traits Mat and Avr (Table S11).
