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We evaluated four additional markers and applied the two most variable, trnH- psbA and nrITS, as supplementary barcodes, and this yielded some improvement in discrimination.
We used AFLP markers, and applied FST outlier (BayeScan, Dfdist) and logistic regression analyses (MatSAM), to identify candidate regions for disruptive selection in the genome and their associations with adaptive traits within each lake flock.
We have created a new open pipeline for developing EST-SSR markers and applied it in a comprehensive analysis of EST-SSRs and EST-SSR markers in C. japonica.
Samples were mixed 1 1 with 10 nm protein A-gold (Aurion, Wageningen, The Netherlands) as fiducial markers and applied to glow-discharged R2/2 Cu 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) by gentle pipetting.
We used the stepwise mutation model for microsatellite markers and applied a MCMC approach, where we calculated 10 short chains (500 used trees out of sampled 10,000) and 3 long chains (5,000 used trees out of sampled 100,000).
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In a discovery-driven approach, we use 2D-difference gel electrophoresis (DIGE) to identify putative clinical response markers and apply power calculations to identify the sample size required to further validate those markers.
Here, we report a large set of BAC-end sequences (BES), use them for developing SSR (BES-SSR) markers, and apply them in genetic linkage mapping.
Vereijken et al. [ 25] obtained a mean accuracy of imputation of about 0.75 in chickens with a low-density panel containing 384 markers and applying Beagle.
These results will be used to develop useful genetic markers and apply them to soybean breeding as well as in broader studies of plant molecular biology and genome evolution.
In line with this approach, Yang et al. recently defined four critical steps in mouse pluripotent reprogramming from initiation to maturation by appropriate markers and applying fluorescence-activated cell sorting (FACS) [ 60].
In addition, the average length of the detected QTL regions became shorter as marker densities increased (Tables 2 and 3), which suggests that QTL regions can be refined by using high-density markers and applying the BVS model together with a QTL intensity profile.
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