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When devising strategies for cloning and expression of heterologous proteins in P. pastoris some points need to be considered from the start, that is, the choice of promoter terminator combinations, suitable selection markers and application of vector systems for either intracellular or secreted expression including selection of proper secretion signals (Fig. 1).
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Tissue markers and their application in process monitoring are presented, and the physical properties that influence the fractionation properties of grain tissues are developed.
The U.S. Environmental Protection Agency EPAA) health research program emphasizes the validation of appropriate biologic markers and their application to high-priority Agency issues.
We previously reported a first generation linkage map with 240 DNA markers and its application to preliminarily map QTL for growth traits in Asian seabass (Lates calcarifer).
This study provided opportunity for the next-generation sequencing of individual Aegilops chromosomes for the development of sequence-based markers and their application in wheat breeding.
The limited amount of sequence information in pearl millet has limited progress in gene discovery and characterization, global transcript profiling, probe design for development of gene arrays, and generation of molecular markers and their application in crop improvement programs.
Yellow mustard has lagged far behind oilseed Brassica species in molecular marker development and application.
It has lagged behind oilseed Brassica species in molecular marker development and application.
The costs of marker development and application are important to consider.
We developed a non-redundant set of unigenes based on all available sequences for pepper (in 2006) to design a tiling Affymetrix GeneChip array for marker discovery and application in pepper [ 11].
Ease in SBP marker development and application to any genomic regions, and genome-wide abundance of SNPs make this technology suitable for mapping experiments, especially to develop high density molecular maps for positional gene cloning experiments, if the assembled genome sequence and physical maps of the studied species are available.
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