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At the end of this step, the BCnF1, which contains the new QTLs at full heterozygosity at all target marker loci, are self-pollinated to fix the target loci (Additional file 5).

Importantly, the λs of the causative and the marker loci are variable but their conferred PAFs remain identical if D'∼1.0.

However, these marker loci are placed on chromosome 5 in the Btau_2.0 sequence assembly.

In our analysis below, we focus on chromosome 3 where six SNP marker loci are genotyped.

Polymorphic marker loci are useful for the construction of such high-density linkage maps.

The LOD scores at 119 marker loci are plotted as small circles.

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Similar(43)

Using these primer sets, 11 new microsatellite marker loci were successfully amplified with unambiguous polymorphic alleles exhibited across 30 individuals examined.

Linkage disequilibrium (LD) between marker loci was assessed and haplotype blocks were constructed using Haploview 4.1.

A consensus sequence of the genome and a set of mapped marker loci were generated.

Allele frequencies at marker loci were calculated in SPLINK [30] for all unrelated individuals in the pedigrees.

In the simulations we manipulated allele frequencies at the two loci, while frequencies at the 498 other marker loci were unconstrained.

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