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The possible use of the GFA protein as a marker for astroglial neoplasia is discussed.
Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia [ 1- 4].
Both F9 and G3 subclones expressed vimentin, a marker for astroglial cell precursors, with lower levels in the FPR− G3 cell subclone.
The cultures used in this study were characterized and validated by immunostaining with GFAP, a specific marker for astroglial cells, and OX-42, a marker for microglia (specific for activated microglia, as the antibody reacts with the CR3 complement).
To investigate whether CoQ10 blocks the activation of astroglial and microglial cells in ischemic retina, we performed whole-mount immunohistochemistry or Western blot analyses using antibodies raised against glial fibrillary acidic protein (GFAP), a marker for astroglial cells, or Iba1, a marker for microglial cells.
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The intermediate filament proteins nestin, vimentin, and glial fibrillary acidic protein was used as markers for astroglial maturation and astrogliosis, and myelin basic protein as a marker for oligodendrocytes and myelin formation.
Cux2 was also detected in Type 2 cells, which express Sox2, but lack a radial glia and markers for astroglial stem cells (white arrowhead, Figs. 3D,J).
There was no sign of neuronal loss and markers for astroglial (GFAP) and microglial (CD68) reactivity were negative throughout the brain providing no evidence of an inflammatory reaction to a more subtle neurodegenerative processes in hFUS or mice up to 12 weeks (Online resource Fig S1).
Next, we evaluated the differentiation of IhNSC-Ps into specific neuronal and glial phenotypes following transplantation into the ischemic environment by analyzing the colocalization of selective markers for neurons, astroglial and oligodendroglial cells with the anti-human specific antibody anti-huN.
Paraffin embedded coronal sections of the brain (12 µm thick) were processed for the immunohistochemical detection of glial fibrillary acidic protein (GFAP) as a marker of astroglial reaction and myelin basic protein (MBP) as a marker of myelinated fibers.
Measurement of protein S-100 is used as a marker of astroglial cell injury.
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