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ChIP-chip analysis of histone H3 acetylation was used as a marker for actively transcribed DNA sequences.
To determine the proliferative status of MHCI- and MHCI+populations, we analyzed the expression of nuclear proliferation antigen Ki67, which is a marker for actively cycling cells.
This suggests that histone H3Lys4 trimethylation serves as a marker for actively transcribed genes, and that the recognition of this marker can lead to different results, including transcription activation or repression.
Ki-67 is the subject of numerous papers in which it is used as a marker for actively dividing cells, but there is very little known about its molecular function.
Its applicability as an activation marker is still unresolved; a semiquantitative analysis of microglia in the SN of PD and incidental Lewy body disease (ILBD) cases compared with controls showed a slight increase in IBA-1 immunoreactivity, but a large progressive increase in CD68, a marker for actively phagocytic microglia [ 28].
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WT or ArtΔ/Δ MSCs were transferred from standard growth to either adipogenic, osteogenic or control medium, cultured for up to 14 days and measured at various culture time points for mitotic index as a marker of actively proliferating cells by immunostaining for phosphorylated histone H3 (phospho-H3), a mitosis-specific marker.
We selected H3K4me3 and H3K27ac as markers for active genes and enhancers, H3K36me3 for actively transcribed regions and H3K27me3 as a mark for transcriptionally silent chromatin, and compared the results with public data.
Of the 4 possible lysine residues (K5, 8, 12, and 16) for acetylated in the H4 N-terminal tail, K16 is the most frequently acetylated in eukaryotes and its acetylated form is a marker of actively transcribed chromatin.
In the present study we investigated whether CD134 can be used as a (transient) marker for targeting auto-aggressive CD4+ T cells in actively induced experimental arthritis.
First, does CD24 actively mediate tumorigenesis, or does it serve only as a surface marker for tumorigenic cells?
Over 50% of the respondents monitored serum ferritin levels quarterly as a surrogate marker for iron overload in their patients, and overall, 38% of transfusion-dependent patients with MDS were actively being treated for iron overload with iron chelation therapy.
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