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Interestingly, LEKTI-2 immunoreactivity was shown to be markedly induced at lesions of clavi (Fig. 8).
Moreover, a G0-specific gene, GAS1, was markedly induced at 24 hr, indicating induction of a G0-like state of quiescence.
The Mk (Mvk1) gene is markedly induced at 4 hours with both methods but remains largely uninduced at the earlier time points (see Figs. 2D and 4I).
Concurrently, stemness-related protein expression, including Abcg2, nestin, and Nanog, was markedly induced at low oxygen tension compared to that at standard 20% O2, with the most abundant protein level found at 5% O2).
At 35°C, the expression of NRKe and 9RKe was markedly induced at 12 h after temperature treatment and then returned to the basal level of expression at 1 d after temperature treatment; the transcripts increased approximately 2- to 2.5-fold at 12 h after 35°C treatment compared to untreated control plants.
Similar(55)
Real-time quantitative PCR and Western blot demonstrated that Egr-1 markedly induced Rad expression at both mRNA and protein levels (Figure 3), which is consistent with Egr-1 transactivation of the Rad promoter.
Over-expression of HGF markedly induced Akt phosphorylation at day 2 (65 ± 35-fold, P < 0.01), and although AdNK4 over-expression in VSMCs for 2 days showed elevated Akt phosphorylation compared to control cells, it was markedly reduced compared to cells induced to over-express HGF (Fig. 3C & D).
We found that although exposure of MDA-MB-468 cells to IR markedly induced DNA-PK phosphorylation at Ser2056, suppression of Cav-1 expression by siRNA effectively inhibited the IR- stimulated phosphorylation of this critical DSB repair factor (Fig. 9A), suggesting that Cav-1 is involved in controlling the activity of the NHEJ pathway.
TNF-α (20 ng/ml) markedly induced degradation of IκBα at 20 minutes, and this degradation was significantly inhibited in cells pretreated with human HSP70.
Our results demonstrated that treatment of PC12 cells with 50 ng/ml of NGF markedly induced miR-16 expression at 2 and 4 days, compared with NGF-untreated cells.
Yet, combination of the two compounds markedly induced p53 and PARP cleavage at the two time points.
Related(20)
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