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The genetic maps of sheep, pig, and opossum have 2371, 462, and 150 markers, respectively.
Our 2-DE gels can be considered the first reference maps of sheep serum.
This study generated the first reference 2-DE maps of sheep serum.
In recent years, low resolution physical maps of sheep MHC class II and III regions have been constructed using a combination of sub-cloning and partial sequencing of Bacterial Artificial Chromosome (BAC) clones known to contain MHC sequences [ 18, 19].
We tested the usefulness of 2-DE maps of sheep serum, and the efficiency of the proteomic approach in demonstrating variations in serum protein patterns during early stage disease or particular physiological states.
This study generated the first reference 2-DE gel maps of sheep serum using pH 3 10 and pH 4 7 IPG strips, and 9 16 T% SDS-PAGE.
Similar(54)
Recently, a physical map of sheep MHC derived from a Chinese Merino sheep has been published by Gao and colleagues [ 30].
Another extensive study published recently by Wu and colleagues [ 16] utilized radiation hybrid mapping to provide a map of sheep chromosome 20 which includes the MHC.
In this study, a new and advanced cytogenetic map of sheep that contains 452 loci and covers almost all of the chromosome bands (mainly R bands) is presented.
In order to characterize normal protein patterns and improve knowledge of molecular species-specific characteristics, we generated a two-dimensional reference map of sheep serum.
Mapping of sheep contigs to human UniGene clusters was performed using blastn with an e-value cutoff of 10-25 as described above.
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