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Mapping of sheep contigs to human UniGene clusters was performed using blastn with an e-value cutoff of 10-25 as described above.
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The genetic maps of sheep, pig, and opossum have 2371, 462, and 150 markers, respectively.
Our 2-DE gels can be considered the first reference maps of sheep serum.
This study generated the first reference 2-DE maps of sheep serum.
Recently, a physical map of sheep MHC derived from a Chinese Merino sheep has been published by Gao and colleagues [ 30].
Another extensive study published recently by Wu and colleagues [ 16] utilized radiation hybrid mapping to provide a map of sheep chromosome 20 which includes the MHC.
In this study, a new and advanced cytogenetic map of sheep that contains 452 loci and covers almost all of the chromosome bands (mainly R bands) is presented.
In order to characterize normal protein patterns and improve knowledge of molecular species-specific characteristics, we generated a two-dimensional reference map of sheep serum.
This study generated the first reference 2-DE gel maps of sheep serum using pH 3 10 and pH 4 7 IPG strips, and 9 16 T% SDS-PAGE.
In the creation of the integrated map of sheep, for example [ 20], comparative data were used only in the local repositioning of loci that had all been FISH-mapped to the same chromosomal band.
We previously constructed a physical map of BAC clone contigs covering the ovine MHC except the autosome insertion region [ 12, 13], and a high accuracy sequence map of sheep OLA was accordingly constructed [ 14].
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