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Exact(5)
We have adapted spPCR for the mapping of transposable elements (both P-elements and piggyBacs) in Drosophila.
Given the success of spPCR in the Drosophila system, adapter-ligation PCR might also be adaptable for mapping of transposable elements.
The ease, efficiency, and efficacy of spPCR could make it a favored choice for the mapping of transposable element in Drosophila.
To facilitate the use of spPCR for the mapping of transposable elements in Drosophila, we have generated a simple and effective protocol for use by the Drosophila community (Splinkerette Protocol S1).
Genome sequences generated using this technology will no doubt facilitate confident mapping of transposable element and retrocopy insertions, as individual reads have the potential to span extensive sequence stretches on both sides of an insertion-genome junction.
Similar(55)
The mapping of all transposable elements found in these parasites shows that repeat coverage is up to three times higher than previously reported.
When mapping to transposable element consensus sequences, up to three mismatches were allowed.
Also, our analysis identified novel miRNA mapping to transposable elements (TE).
RepeatMasker tables at the UCSC Genome Browser (hg18) were utilized to map the coordinates of transposable elements into the genomic region of X chromosome.
We also determine multiple genomic clusters of 24-30 nt Piwi-interacting RNAs (piRNAs) that map to transposable elements (TEs) and 3'UTR of protein coding genes.
The mycelia library was also enriched for small RNAs that mapped to transposable elements.
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