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Compared with the entire chromosome 3A shotgun assembly, mapped contigs were significantly enriched for genic sequences.

The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb).

When 2,581 partially mapped contigs were analysed, the identity was 99.90% over a length of 37 to 1,900 bases.

Following the initial alignment, mapped contigs were filtered (using the delta-filter utility) to achieve a one-to-one mapping of the contig query sequences to the reference chloroplast genome.

The cumulative length of mapped contigs were 138.3 Mb, representing 53.6%% of the chromosome arm, exceeding that of 1BL physical map at 48%% [ 16], despite lower coverage of the chromosome arm by the overall map.

The chromosomal locations of the remaining mapped contigs were similarly confirmed (Supplementary Table S11).

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Similar(51)

Thirty-six optical map contigs were constructed for the corresponding known 36 chromosomes of the Leishmania major Friedlin genome.

Therefore, if the map contigs were reliable, the positive BACs screened with a single-copy marker should originate from the same region of the genome and all be assembled to a segment of a single contig based on the overlap among clones [1], [32].

Based on these marker-associated BACs, a total of 931 physical map contigs were linked with the linkage map.

The positions of physical map contigs were determined in the linkage group based on the locations of BES-associated markers.

The results of all of the approaches together have verified that the physical map contigs were assembled properly.

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