Sentence examples for mapped sequences were from inspiring English sources
Sequencing reads for two- and four-barcode libraries were aligned to the Saccharomyces cerevisiae genome (build SGD1.01) using Bowtie [8], run with the default options and -m 1, and mapped sequences were viewed in the SeqMonk genome browser (http://www.bioinformatics.bbsrc.ac.uk/projects/seqmonk/).bbsrc.ac.uk/projects/seqmonk/
These mapped sequences were assumed to be noncoding WCR RNA.
Unmapped or redundantly mapped sequences were removed from the data set, and only uniquely mapped sequences without any mismatches were used for further analyses.
Thereafter, the clear reads were mapped to Citrus clementina genome (JGI version 0.9, http://www.phytozome.org/clementine.php, 35976 sequences) using SOAP, only perfectly mapped sequences were retained and analyzed further.
Thereafter, the clear reads were mapped to C. sinensis genome (JGIversion 1.1, http://phytozome.jgi.doe.gov/pz/portal.html#!info alias=Org_Csinensis) using SOAP, only perfectly mapped sequences were retained and analyzed further.
Mapped sequences were visualized by JBrowse and Integrative Genomics Viewer (IGV) [ 46].
The identity of mapped sequences is 98.14%, which shows a close genetic relationship between B. juncea and B. rapa (Additional file 1: Table S1).
Mapped sequences are those with retrieved GO terms associated with the hits obtained after a BLAST search.
To control for inhomogeneity of the magnetic field, field map sequences were realized before the EPI sequence.
Sequences were mapped against reference sequences for splice variants AID-FL, AID-ΔE4, AID-ΔE4a, and AID-ivs3 and uniquely mapping sequences were quantified using CLC Software (Version 6.0.1; performed at GATC Biotech, Germany).
However, improvements of the coding efficiency are still possible, taking into account the fact that the video and the depth map sequences are strongly correlated.
