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Exact(5)

However, many of the short reads obtained from the foot printing experiment will be discarded if they map to repeat regions.

In both stages, every valid alignment to the reference genome is reported, including all information about how reads map to repeat sequences.

These probe sets contain either a single probe or overlapping probes and furthermore, almost all probes map to repeat masked regions and thus were left out in the new chip definition.

Repeated elements, which are estimated to cover 42.4 % of the apple genome [ 16], create a problem for read-depth analysis, as reads which map to repeat regions of the reference create noise in the read-depth signal.

Thus, although the overall numbers of small RNAs that associate with EhAGO2-2 and map to repeat or retrotransposon elements is low, they may play a functional role in controlling genome stability as has been shown in other systems [ 47, 48].

Similar(55)

After mapping of reads and removal of duplicate mappings (i.e. reads mapping to repeat regions like rRNAs), 15.96 and 2.65 million reads were mapped uniquely to the genome from the whole and the primary transcriptome library, respectively (Table  1).

The data indicated that about 16 21% of the small RNAs matched with rRNAs, tRNAs, snRNAs and snoRNAs (Figure 2C and 2D), ∼24% mapped to repeat sequences, including transposons and retrotransposons, and ∼1.2% mapped to protein-coding exons.

Subsequently, the percentages of reads mapping to repeat families were calculated (Table 3).

Of the SNPs identified, 1,832,801 (53%) mapped to repeat containing regions of the genome [ 27, 28].

Consistent with earlier studies, we found that a large proportion of all aligned reads mapped to repeat elements.

After removal of the reads that mapped to repeat regions, including the rRNAs and tRNAs, the remaining 711,851 reads were uniquely mapped either to ORFs or IGRs.

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