Sentence examples for many restriction sites from inspiring English sources

Many restriction sites are present only once or twice in the sequenced fragment, making our dataset lowly informative about the association of ISR with particular sequence motifs.

We expect sufficiently large sequences with many restriction sites to match uniquely to the optical map.

Since many restriction sites are conserved across a species, the sequenced portion of the genome is highly consistent within a population.

For other applications such as cloning, restriction endonucleases are used to great effect, however, a restriction site may not be conveniently located, or, too many restriction sites may exist within a defined sequence.

The multiple cloning sites of pHD-DsRed-attP were designed to conserve as many restriction sites as possible from pGX-attP to make the vector compatible with homology arms previously generated for ends-out homologous recombination (Huang et al. 2009).

The application of restriction-based protocols at taxonomic levels beyond that of populations, or perhaps subspecific taxa, entails a greater risk due to the possibility that many restriction sites may possess mutations in a subset of the study samples, resulting in frequent allele dropout and, at worst, data matrices consisting largely of missing data.

Then we check how many restriction cutting sites are around this probe within these L bases.

The mobile genetic elements are, however, relatively short sequences (see Additional file 1: Tables S3 and S4) and may lack many restriction cleavage sites of restriction endonucleases.

However, the Ubi promoter contains many frequently used restriction sites, such as BglII, EcoRI, SalI, XbaI, and XhoI (Additional file 1: Figure S1).

At the same time, we considered that the paucity of genus-associated restriction sites in many genomes [ 23, 24] and the rapidity with which genes encoding restriction enzymes are gained and lost in organisms [ 25] might point to underrepresented oligonucleotides as a source of a genome signature that changes rapidly over evolutionary time.

Alternatively, many SNPs lead to polymorphisms in restriction sites which can be used to develop Cleavage Amplified Polymorphism (CAPs) markers.