Sentence examples for multiple restriction sites from inspiring English sources

Finally, we modified pMTL007 by introducing the upstream fragment of operon CAC1493-1494 (626 bp, named H1) as homologous allele into the downstream region of the eythromycin resistance gene (Ermr), the 3'-terminal sequence of intron (1907 to 2099), where multiple restriction sites including PmlI and SalI exist (Fig. 2).

There is no evidence of germline transmission, no evidence of functional contribution, and uncertainty about the contribution of multiple restriction sites to the generation of the reported fragments.

All channel subunits were subcloned into the oocyte expression vector pBF, which provides 5′ and 3′ untranslated regions from the Xenopus-globin gene flanking a polylinker containing multiple restriction sites.

The "targeting plasmid," pSV001, was generated by ligating a synthetic DNA fragment carrying multiple restriction sites, an I-SceI recognition site, and an F3 recombination site (Integrated DNA Technologies) in pFL44S{w+}-attB linearized by KpnI and XbaI and treated with "Alkaline Phosphatase, Calf intestinal" (New England Biolabs).

Among the restriction enzymes, some combinations of enzymes can be used for the digestion of multiple restriction sites at once in the same reaction buffer; for examples restriction enzymes SacI and KpnI can be jointly used in the low salt buffer, EcoR I, Hind III and TaqI can be jointly used in the medium salt buffer, and BamHI, ClaI and PstI can be jointly used in the high salt buffer.

Degenerate contigs originating from the vectors used for library preparation and sequencing were removed using Blast VecScreen (http://blast.ncbi.nlm.nih.gov, using the UNIVEC database), after confirmation of the presence of the vectors' multiple restriction site segments (polylinkers) from "Dynamic restriction site" in CLC.

Inspection of the 28 P. dominulus microsatellite sequences described by Henshaw [26] revealed that 23 consisted of multiple cloned inserts (as they contained multiple GATC restriction sites).

The array of multiple restriction endonuclease sites provides versatile possibilities for the choice of the genomic restriction fragments utilized and for the final linearization procedure prior to the delivery into ES cells.

In the construction of C-terminally truncated topoIIβ, multiple internal restriction sites in the topoIIβ sequence meant that a complex cloning procedure was necessary.

The chromosome of JCM1046 has multiple SmaI restriction sites, while pCTN1046 has none.

Due to the presence of multiple HindIII restriction sites into the CDCA7L-promoter, a three sequential steps construction was performed.