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How many replicates, and how do we assess significance?
Details of how many replicates and statistical analyses used to discern differences should be provided.
Summary: With their many replicates and their random layouts, Illumina BeadArrays provide greater scope fordetecting spatial artefacts than do other microarray technologies.
This would result in a high computation time that would be hard to manage in a simulation study due to the many replicates, and memory problems.
As ABC estimation needs many replicates and thus a huge computer power, we decided to perform the estimation of parameters only under the best scenario(s).
Because of this variability, detection of a significant difference in the function of NAF and CAF required many replicates and multiple fibroblast cultures derived from different individuals.
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Sea-based mesocosms might yield more potential to use many replicates than land and indoor facilities, and therefore a better statistical evaluation of the results.
Notwithstanding this redundancy, the 9,534 clones were used in the microarray construction for two reasons: (i) this approach was less error-prone than attempting to select a unique clone set and (ii) this in-built redundancy provides many replicates of individual contigs and clusters, which can be exploited in quality-control analyses.
Consequently, large population sizes and many replicates can only be achieved in evolution studies with simple maintenance and rapid phenotyping.
This experiment used a prolonged protocol of many replicates within each patient, and was specifically designed to detect differences in their haemodynamic implications of different definitions of AV and VV delay.
How many independent EVC lines should be included to test for transformation effects, and how many replicates are necessary to detect significant ecological effects of single genes?
Related(20)
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