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This method allows incorporating multiple replicates and possibly multiple reference ('housekeeping') genes for estimating relative expression and corresponding confidence interval.
Many interspecific crosses were represented by multiple replicates, and in these cases, we calculated the mean for each measure of reproductive isolation and used these mean values in subsequent analyses.
In a second step, we assessed the number of times a given barcode was significant across multiple replicates and multiple arrays, again using the criteria of equal rates of false positive and true positive loss (Figure S2C).
Furthermore, microarray experiments are less time consuming, allow the run of multiple replicates and established analysis platforms for routine transcript level analyses are available.
Since the experiment in Fig. 1F was less representative of multiple replicates, and for reasons described in point #4 below, we removed this panel.
Using Illumina MouseRef-8 BeadChip arrays, multiple replicates, and carefully controlled culture conditions, highly significant (p < 0.0001) expression data for differences as low as 1.2-fold were achieved.
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The prediction accuracy achieved was remarkably high because of the uniform levels of variance across the multiple replicates analysed and because of the high correlation coefficients between replicates (the average ρ ≅ 0.92).
Cell suspensions (at 2 × 104 cells/ml) were transferred to 96-well plates in multiple replicates (×5) and incubated for 3 days with different drug's concentrations (ranging between 1 nM and 100 μM).
An ideal study system to address the ecological and evolutionary consequences of color polymorphisms would incorporate multiple replicates, sex-independence, and a simple genetic basis of the color polymorphism associated with assortative mating.
Future research using multiple replicates of sympatric and allopatric pairs will determine the extent of local adaptation in this system.
Accuracy of microarray-generated data can be improved by using multiple replicates, dye swaps and statistical data analysis.
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multiple captures and
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multiple chins and
multiple commandos and
multiple campaigns and
multiple dogs and
multiple layers and
multiple calls and
multiple canoes and
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multiple voices and
multiple cameramen and
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