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In addition, TBLASTN analyses were manually validated to take into account a gene having a frameshift mutation.
The computation was then manually validated to exclude possible errors.
This automated algorithm was manually validated to ensure accurate quantitation and partner assignment.
The assigned ions were manually validated to ensure the quality of assignments.
All MS2 and MS3 spectra from novel ORFs were all manually validated to confirm both identifications and quantifications.
TBLASTN analyses were manually validated to take into account genes with frame-shifts due to sequencing errors.
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Images were manually validated according to the following: 1) >10% of the tissue area is pan-cytokeratin positive, 2) >50% of the image was usable (that is not compromised due to overlapping or out-of-focus tissue).
This automatic annotation was then manually validated so as to correct inadequate labeling and hence avoid noise in the processing of the coherence dimensions.
We manually validated the peptides to identify the phosphorylated residues.
For 2 proteins of B. abortus 2308, we report the expression of manually validated peptides corresponding to the sequence found upstream of their currently annotated start sites.
All AMPs in DAMPD database were manually curated and experimentally validated to possess antimicrobial activity.
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