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All files were manually confirmed to be duplicates.
When files have been manually confirmed to be redundant, only one representative of the group is kept.
The deduced amino acid sequences of candidate nsLTP genes were manually confirmed to harbour the 8 CM (C … C … CC … CXC … C … C, where X represents any amino acid) and genes lacking the motif and encoding for the putative proline-rich proteins were excluded from further analyses.
Given the child's first and last name, birth date, and sex; mother's first and last name and birth date; and father's last name and birth date, we probabilistically matched the two records and reviewed all high scoring linkages (≥ 25), almost half of the linkages (9,120 of 22,806), only accepting those manually confirmed to be likely matches (see CDC for record linkage concepts) (CDC 2010b).
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This is in contrast to the CellSearch system, in which images of cells are determined to be CTCs by software and need to be manually confirmed by an operator.
All SD-OCT segmentations were manually confirmed and corrected as needed to follow the internal limiting membrane and Bruch's membrane prior to computation of SD-OCT change maps and CST (MEM, CCW).
The best-matched spectrum for individual glycopeptide in different peptide backbones was manually confirmed and used as spectral template to cross-match the putative glycopeptide spectra using b-, y-, peptide, Y-ions, and software Xcalibur version 2.2 (ThermoFisher Scientific).
To check the accuracy, we manually confirmed whether these genes are associated with the essential hypertension.
Hits to these 395 peaks were then manually confirmed.
As all these molecular associations were manually confirmed by reading the individual full published abstract, and thus these molecular associations can be trusted to be highly reliable.
All selected spots were manually confirmed and statistically evaluated (p < 0.05) upon Student's t-test, using XLSTAT (Addinsoft, France, add-in to Microsoft Excel).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com