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Each seed was manually dissected to extract the haploid, maternally inherited megagametophyte tissue.
Tissue epithelia were manually dissected to enrich for cells of cancer origin and to obtain samples with maximally homogenous protein composition (Appendix Fig S1).
All crude tissular explants were manually dissected to obtain separate samples of each tissue type (that is, cartilage, synovial membrane and subchondral bone).
The lobes were manually dissected to produce groups of 5 10 oocytes and incubated in OR2, containing 1 mg mL−1 collagenase from Clostridium histolyticum (SIGMA, Sigma Aldrich).
Cases were reviewed by a histopathologist and if deemed to have <50% malignant crypts in the section, the tissue was manually dissected to ensure a high proportion of tumor cells.
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Spontaneously contracting areas were manually dissected at day 20 30 of differentiation.
Following hybridization, embryos were manually staged and dissected to examine neurexin expression.
In brief, 2 mm skin punch biopsies were manually dissected into five to ten tissue pieces and transferred to 1.5 ml medium in a 25-cm cell culture flask (BD Falcon, Heidelberg, Germany).
This was performed to compare the host expression profiles obtained from the more heterogeneous manually dissected lung tissue, to the expression from host tissue surrounding the colonies of A. pleuropneumoniae.
When these cells were manually dissected and transferred to HFF feeder cells and cultured in KO-SR medium, colonies resembling hESCs (Figure 4A (e)) were formed at an efficiency of 1.35 to 2.07×10−4% (from 5 independent experiments).
From the sex separated and age pooled animals about 1000 antennae, 600 legs, 150 mouthparts (as piece of the head capsule anterior of the antennae), 50 heads (the whole head capsule excluding the antennae) and 20 bodies (excluding head and legs) were manually dissected and immediately transferred to ice cold RNA lysis buffer (Zymo Research, Irvine, USA).
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