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Until recently, development of live cell imaging probes relied mostly on qualitative, manual microscopic observations of stained cells.
However, manual microscopic sediment examination is labor-intensive, time-consuming, and lacks standardization in high-volume laboratories.
In this study, the concordance of analyses between manual microscopic examination and two different automatic urine sediment analyzers has been evaluated.
The coincidence between the results of the proposed automatic method and manual microscopic expert scoring is ambiguous.
BAL cell type composition was determined by manual microscopic analysis of stained cytospin preparations of BAL fluid.
Hence, manual microscopic blood examination should always be used to validate the automated methods as previously suggested by Lantis et. al[ 1].
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The purpose of this study was to evaluate the quantification of fetomaternal hemorrhage by the manual and automated microscopic analysis of Kleihauer-Betke stained slides and by flow cytometry.
Samples were subjected to microscopic (manual) differential cell counting of leukocyte types if flagged for this during the initial automated differential counting.
None of the KO masses within this cohort were detectable by manual palpation, but microscopic analysis of H & E-stained sections revealed lesions < 5 mm in five of eight recipients.
In order to reduce the reliance on manual assessment of microscopic findings, we set out to develop a fully automated method to score DSS-induced colitis in mice by using image analysis software, Definiens.
Upon manual examination of microscopic images, 21 duplicate compounds (occurring in more than one library), seven obvious false positives (DNA stains) and 45 compounds with low quality staining (few cells left in well, background, indiscriminate whole cell staining) were sorted out.
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