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To analyze the gene expression data in an unsupervised manner, we carried out principal component analysis (PCA).
In order to evaluate jiadifenoic acids C in a more systematic manner, we carried out a series of experiments.
To assess copy number profile agreement between the two platforms in a ploidy-independent manner, we carried out hierarchical clustering of the sample profiles using a Pearson correlation distance metric.
Since secreted CLCA1 can activate TMEM16A-mediated ICaCC in a paracrine manner, we carried out similar imaging experiments to determine whether exogenously applied secreted CLCA1 also increased TMEM16A surface expression.
For tracking cyt. c release and its dependency on caspase-3 in a real time manner, we carried out live-cell imaging in endogenous caspase-3-expressing SW480, caspase-3-deficient MCF7 and caspase-3-expressing MCF7 MCFC3) cells (Supplementary Videos S1, S2 and S3; Figures 1a c).
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To enhance Met signalling levels in a temporally and spatially regulated manner, we generated mice carrying a conditional mouse human chimeric met transgene (met tg ).
As all of us begin to immerse ourselves in the 2012 presidential election campaigns -- be it the unpredictable Republican nominating process or the intense and expensive general-election contest to come -- maybe we can communicate in a manner that reflects how we carry ourselves in our lives outside of politics.
If her subjects didn't know who she was, they'd be undone by her little-old-lady manner (she carried her camera in a wicker shopping bag, had no lights), which she'd long ago perfected.
Passivation of the nc-Si−SiOx structures, obtained in this manner, was carried in an aqueous solution of the HF.
An additional statistical analysis in a "frequentist" manner was carried out in order to quantify the probability of this mutation being a null finding.
Showing respect and preserving dignity were major concerns for the professional caregiver and she took this into consideration in the manner she carried out tasks.
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Justyna Jupowicz-Kozak
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