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Consequently, inhibition of acid secretion is now considered to be mandatory for proper assessment of intraluminal PCO2.

It should be highlighted that the use of image fusion was mandatory for proper diagnosis in all cases.

However, we cannot neglect the usage of cryo-protectants, as these substances are mandatory for proper vitrification of mammalian cells.

Using the CD45 marker in tumor tissues is therefore mandatory for proper analysis of leukocytes, together with other specific antigens in the case of hematological tumors.

NS3 catalytic activities depend on formation of a tight, non-covalent complex with the HCV NS4A protein, a cofactor mandatory for proper protein folding and stabilization of the protease in proximity of cellular lipid membranes (Lindenbach, 2013; Lohmann, 2013).

Experimental evidence in CHO cells demonstrated that the simultaneous synthesis of the four sarcoglycans is mandatory for proper assembly and cell membrane localisation of the sarcoglycan complex (Ref. 82).

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Surgical created subperiosteal and endosteal scaffolds for bone augmentation have to be perceived by the clinician more as "fracture sites" similar to "natural" fractures of the jawbones and its mandatory immobilization for proper bone healing.

Conceptually, our findings unravel the complexity of gene regulation, as regards key genes involved in the heterocellular cell adhesion between developing thymocytes and the major component of the thymic microenvironment, an interaction that is a mandatory event for proper intrathymic T cell differentiation.

However, it is widely accepted that an aggressive rehabilitation protocol is mandatory for a proper ROM recovery and to avoid the onset of arthrofibrosis and heterotrophic ossifications.

In fact, due to their reduced dimensionality, the inclusion of many-body (MB) effects in the theoretical description, in the so-called many-body perturbation theory (MBPT), is mandatory for a proper interpretation of the excited state properties.

Correctly quantifying the contribution of each heating mechanisms is mandatory for a proper design of the nanoparticles as a function of the application in which they are supposed to be used.

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