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And I watched as the males, separated from the females, glanced at the women as they entered.
Bacterial growth was measured in two treatments: virgins and males separated from females four days prior to infection.
This experiment was repeated in two more blocks with each block containing three treatments - (a) virgin males, (b) males separated from females for one day (following a two day interaction period) before infection and (c) males separated from females for four days (following a two day interaction period) prior to infection.
We repeated this experiment in two more blocks where we had two treatments: 1. males separated from females for four days (after two day interaction with females) before infection and 2. Males separated from females for 1 day (after two day interaction with females) prior to infection.
Males kept in a pen with only females have also been observed challenging males separated from their pen by a 1 m wide corridor by performing frontal marking on bushes and small branches, and frontal marking on the wire fence right in front of the place where another male would be standing.
However, the number of CFUs in virgins and mated males separated from females for four days was not significantly different indicating that the ability to resist Pe in mated males is a temporary effect which declines if further access to females is denied.
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To examine the courtship enthusiasm of Gr68a males separate from their tracking ability and performance quality, we employed a decapitated immobile wild-type female as a courtship target in a 50 mm2 chamber.
The males and females were allowed to interact for two days, after which the males were separated from the females (under light CO2 anesthesia) on 14th day post egg collection.
Males were separated from the population the following day, and acclimated in the treatment aquaria for 48 h.
At the end of the 2-month-cohabitation, the males were separated from the mating females and the females were observed for 3 more weeks for pregnancies that occurred during the last period of mating study.
Two females were housed together with 1 male for one week after which the male was separated from the females.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com