These morphological changes occur asynchronously across the retina over 2 3 days; by 7 d.p.f. the vast majority of cones are gone in the mutant retina.
Notably, by P21, Sall3 immunostaining was more prominent in cones of the medial and ventral retina, where the great majority of cones express Sop, as compared with the dorsal retina (Haverkamp et al., 2005; Szel et al., 1993).
However, the large majority of cones displayed, similar to rods, responses to the first dim G flash, a plateau after the brighta G flash, and a slow recovery of the dim flash response.
Coexpression of Sall3 and Mop was most prominent in dorsomedial and ventromedial retina, where the overwhelming majority of cones coexpress both Mop and Sop (Onishi et al., 2010a; Szel et al., 1993).
The vast majority of cones imaged at 1.5° and 3° retinal eccentricity and a smaller but still appreciable fraction at 6° retinal eccentricity generated two distinct reflections that straddled the cone OS, one at the IS/OS junction and the other at the OS posterior tip.
13 After automated identification of the majority of cones in the image, the observer then reviewed each image and manually identified cones they deemed to be missed by the algorithm or removed cones they deemed to be selected in error by the algorithm.
