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The nanostructures in sample 1 maintained their stem size during growth, and only a small segment of the stem near the terminal particle exhibited a decreased dimension because of the relatively low In vapor saturation toward the end of the experiment.
Some cells differentiated into neurons, while others maintained their stem cell phenotype.
When cultured in a serum-replacement medium, the spheres maintained their stem markers for months.
During subculturing, the NSC culture maintained their stem cell characteristics and stained positively for Nestin and SOX2 (Supplementary Figure S1B).
The authors were able to demonstrate that some of the cells remained within the lung and maintained their stem cell phenotype.
Furthermore, characterization of the expanded population by immunofluorescence microscopy and flow cytometry showed that NT2 cells maintained their stem cell characteristics along the bioreactor culture time.
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To investigate the availability of iPSCs as bioengineered substitutes in corneal repair, we developed a thermo-gelling injectable amphiphatic carboxymethyl-hexanoyl chitosan (CHC) nanoscale hydrogel and found that such gel increased the viability and CD44 + proportion of iPSCs, and maintained their stem-cell like gene expression, in the presence of culture media.
The MSCs that were used in this study were immortalized by the ectopic expression of human telomerase reverse transcriptase (h-TERT), which increased the life span of the cells [ 24] and maintained their stem-like properties [ 30].
Bulge cells maintain their stem cell characteristics after propagation in vitro, thus ultimately they may be useful for tissue engineering applications.
Following an injury, satellite cells proliferate and incorporate their nuclei into existing fibers while still maintaining their stem cell population.
First considerable factor is the optimal cryopreservation media in which cells can maintain their stem cells abilities for long time.
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Justyna Jupowicz-Kozak
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