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We also showed that MSCs could preserve their stem cell nature upon microencapsulation and easily be localized with retained viability upon in vivo implantation.
Methodology for the cultivation of LEC on AM to preserve their stem phenotype and encourage proliferation and stratification continues to be rigorously investigated [5,10].
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However, a major problem remains in clinical practice, which is the question of whether stem cells preserve their self-renewal and differentiation potential in the culture conditions or not.
For younger boys, research is under way to see whether freezing sperm-forming stem cells will preserve their future fertility.
Evidence from mammalian system has demonstrated that Fbxw7, an F-box protein subunit of an SCF-type ubiquitin ligase complex, targets positive regulators of the cell cycle including Cyclin E, c-Myc, Notch, and c-Jun, and maintains mouse hematopoietic stem cell quiescent to preserve their capacity for self-renewal (Onoyama and Nakayama 2008).
An original procedure and optimal hydrogel recipes were developed to encapsulate fibroblasts and adipose-derived stem cells, while preserving their viability and proliferative ability during ex vivo temporarily storage.
Interestingly, HA can substitute hypoxia for the long-term maintenance of embryonic stem cells in culture, preserving their pluripotency and thereby providing a useful alternative that enhances their viability [ 67].
In this study, we obtained epithelial differentiation of SP cells without shifting the cells to standard differentiating conditions (add 10% FBS in medium) but rather by maintaining the cells under non-differentiation conditions (which preserves in vitro their stem-like properties) in the presence of sesamin.
Cut at the base of the stems to preserve as much stem as possible.
DOI: http://dx.doi.org/10.7554/eLife.03363.002 Quiescent stem cells preserve the stem cell pool for activation following disease or injury.
These physiological changes in the Indy mutant midgut preserve intestinal stem cell (ISC) homeostasis and are associated with healthy aging.
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