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All mice were maintained in ventilated microisolator cages (Animal Care Systems, Centennial, CO) in the University of Maryland animal facility.
Animals were maintained in ventilated cages under standard conditions of 12 hours of light, 12 hours of dark, with water and commercial rodent chow provided ad lib.
Mice were bred and maintained in ventilated cages under specific pathogen-free conditions in the University of Texas Health Science Center at San Antonio Laboratory Animal Resources Department, an AAALAC-accredited facility.
Six- to eight-week old nu/nu athymic male mice were maintained in ventilated cages and fed/watered ad libertum and the experiments were be carried out under an IACUC approved protocol as well as following institutional guidelines for the proper and humane use of animals in research.
Male wild-type (WT) and RasGrf1 −/− mice were maintained in ventilated cages and survival function was estimated from lifespan data.
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Rats were maintained in individually ventilated cages (Tecniplast) under standardized conditions (at a temperature of 21±2°C, a humidity of 65±5% and with an artificial 12 h light/dark cycle) with free access to standard chow (0.25% sodium; SSNIFF) and drinking water ad libitum.
All animals were maintained in individually ventilated cages.
Mice were maintained in individually ventilated cages (IVC) (Allentown Inc., NJ, United States).
Rats were maintained in individually ventilated cages (4 per cage) in the Animal Holding Facility (AHF) at the International Medical University (IMU) after their arrival.
SCID beige mice were maintained in individually ventilated cages, received autoclaved food, water and bedding according to the institutional guidelines under a UK Home Office project license.
Female BALB/c mice, 6 8 wk of age and maintained in individually ventilated cages, were infected with 200 H. polygyrus bakeri infective L3 larvae using a gavage tube.
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