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When vole TS-like cells are maintained in differentiated conditions for 12 to 14 days, they lose Tsix expression completely, most likely due to repression of the Tsix promoter on the active X chromosome (Fig. 5A, lane 5).
As shown in Fig. 4B (upper panel), we obtained two independent clones of pre-adipocytes exhibiting a very strong down-regulation of DNA-PKcs expression and we assessed that this down-regulation was further maintained in differentiated adipocytes (Fig. 4C, upper panel).
The expression of COUP-TFs was maintained in differentiated CMs at day 31.
CD166 was spontaneously lost in undifferentiated cells whereas its expression was maintained in differentiated UCMSCs.
Lineage-specific DNA methylation patterns, which are established during embryonic development, are in general faithfully maintained in differentiated adult cells.
DNA methylation patterns are established during embryogenesis and then faithfully maintained in differentiated tissues, enabling preservation of cellular identity through multiple somatic divisions.
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Although PRH expression is generally down-regulated during terminal differentiation of haematopoietic cells [ 14, 58], PRH expression is maintained in terminally differentiated granulocytes [ 14].
To investigate whether this expression is maintained in terminally differentiated multinucleated osteoclasts, PBMC adherent cells were cultured for 8 days in the presence of M-CSF and further differentiated by exogenous addition of RANKL for 6 days.
Further, the adhered cells were maintained in a differentiated state for 7 days and neuronal network formation and expression of neuronal markers were investigated using fluorescence microscopy, SEM and immunofluorescence.
The level of DNA-PKcs expression was further maintained in the differentiated adipocyte (D7 and D14).
Intriguingly, even though levels of albumin expression were lower in all types of HFH gels compared to the Huh 7.5 gel (Fig. 7G), HNH4α expression in the HFH gels containing additional mLBM, HGF-HUVEC or both components (Fig. 7H) approached levels found in the Huh-7.5 gel, perhaps indicating that some HFH were maintained in a differentiated state.
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