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Calcium phosphate-coated scaffolds induced the expression of osteocalcin by hOBs when maintained in osteogenic conditions.
The results showed that MSCs maintained in osteogenic medium, secreted factors at specific time points that induced alkaline phosphatase activity (ALP) in exogenous MSCs as well as their migration.
To assess the level of mineral deposition, hMSCs maintained in osteogenic differentiation medium were stained with an Alizarin red solution on day 16.
An equal number of samples were maintained in osteogenic conditions but did not undergo Raman analysis.
For all experiment periods, cells were maintained in osteogenic media, and media was changed every three days.
Briefly, MG-63 cells were seeded at a density of 1 × 10 cells/mL onto the surface of PCL fibers and surface-modified PCL fibers into a 24-well tissue-culture plate and maintained in osteogenic medium (DMEM supplemented with 10% FBS, 50 μg/mL ascorbic acid, 10 nM dexamethasone, and 10 mM β-glycerophosphate in the presence of 100 U/mL penicillin and 100 μg/mL streptomycin) for 10 days.
Similar(54)
After being maintained in an osteogenic medium for 2 weeks, the scaffolds seeded with hUCMSCs were implanted into the calvarial defects.
Using the redox ratio, calculated from the contributions of NAD(P H and flavoproteins, we identify distinct patterns in the evolution of the metabolic activity of hMSCs maintained in either propagation, osteogenic or adipogenic differentiation media.
The Alginate-PKH67-labeled MSCs composite was then maintained in a 1 1 mixture of the osteogenic medium and incubated under conditions identical to those used for the preparation of the Alginate-MSCs composite.
The Alginate-MSCs composite was maintained in a 1 1 mixture of the osteogenic medium (DMEM-LG containing 10% FBS, antibiotics, 100 μM ascorbate-2-phosphate, 10-7 M dexamethasone, and 10 mM β-glycerophosphate) and incubated at 37°C in a humidified atmosphere of 5% CO2 and 95% air for one additional week.
Afterwards all samples were either maintained in control medium or replaced with chondrogenic or osteogenic medium for 28 days.
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