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Stimulation (square pulses, 1 ms duration, 1 Hz, 2.3 or 4.6 V/cm) was initiated 24 h after cell seeding and maintained for additional 72 h.
(A) Representative TEM images of HeLa cells treated with 0.25 µmol/L Doxo for 24 h and maintained for additional 24 h with 0.5 µmol/L Doxo in the presence or absence of 5 µmol/L SVT016426.
After immunization, all mice were maintained for additional 7 weeks.
After 2 h, gel was formed and the reaction was maintained for additional 12 h (conversion: 90 % by GC).
After incubation, the medium was removed and replaced with fresh medium and cells were maintained for additional 24 h.
The culture was maintained for additional 18 days to match the duration of infected ductal sphere maturation.
Similar(52)
Preventive measures described above for surfaces, kitchenware and food were maintained for 2 additional weeks, and no additional cases were detected after 1 month had passed since the first case.
The effect on TAFGC values was maintained for an additional 2 weeks after cessation of treatment.
If no changes are observed after 20-25 min, a stressor drug, usually nitroglycerine or isoproterenol, is administered and the orthostatic challenge is maintained for 15-20 additional minutes.
Controlled haemorrhage (45% of estimated blood volume) was induced over 15 minutes and sustained shock (MAP target 40-45 mmHg) was maintained for an additional 75 minutes.
In both groups, IAP was maintained for 1 additional hour at 20 mm Hg; but in the second one, MAP was increased about 20 mm Hg by means of norepinephrine to reach APP >60 mm Hg.
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