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The improvement gained through the previous steps needs to be maintained for continuous success of the organization.
In this experiment, the cells were maintained for continuous replication.
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Uniform-sized hEBs produced by manual passaging using a 1∶4 split ratio have been successfully maintained for over 20 continuous passages.
Dose adjustment may be required in the presence of reduced creatinine clearance, but the dosage should be maintained for patients undergoing continuous venovenous hemodialysis.
The same temperature is maintained for 2 h with continuous stirring, and then, it undergoes natural cooling for 8 h.
A Zn(NO3 2 solution (2.5 mL, 0.1 M) was then added to the solution, which was continually stirred for 5 min. Then NaOH solution (40 mL, 0.1 M) was directly added to the above solution dropwise, and the resulting mixture was maintained for 30 min under continuous stirring.
Its beneficial effect is maintained for one year of continuous monthly administration and at least for 6 months after the administration is discontinued.
These changes were maintained for over 2 years in continuous culture, indicating their long-term effects.
Accumulation of p16INK4a was detected in the WMM1175_p16INK4a cells 24 h post-induction, and this was maintained for the 5 days of continuous IPTG exposure (Fig. 1A).
Hence, the genotype of continuous cell lines can change with time and cell lines should not therefore be maintained for extended periods of time in continuous culture (Wenger et al, 2004; Saito et al, 2011).
Benefitting from synergies in cooperative structures and maintaining flexibility for continuous innovation processes seem to be crucial factors in very different contexts.
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