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Tobacco (Nicotiana tabacum cv BY-2) and Arabidopsis thaliana (var Lansberg erecta) suspension cell cultures were maintained and prepared for biolistic bombardment as described previously [60].
IOSE-64 hTERT cells were maintained and prepared as described [ 34].
It was maintained and prepared for use in the ISR assays as previously described [ 32].
KSH designed the study and protocols, performed animal experiments, immunostained tissue, collected images, maintained and prepared cells for transplantation, executed the data analysis, and prepared the manuscript.
Cells were maintained and prepared for experiments as described previously (Nagy et al. 2002), except that cells were plated at a concentration of 7.5 × 10 cells per well and sera were applied in 10-μL aliquots [see Supplemental Material (doi 10.1289/ehp.0901113)].
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Each rider maintains and prepares his own rope, which can act as a lifeline, far left, when things get wild in the ring.
But only this election did he learn the extent of centralisation in the organisations that are in charge of maintaining and preparing the voting machines.
14 Intestinal epithelial cultures were isolated and cultured as described in Sato et al. 15 Colorectal tumour cell lines were maintained, and cell lysates prepared, as described in Emaduddin et al. 16 For de-novo methyltransferase inhibitor treatments, SW48 cells were plated in six-well plates at 2×10 per well.
Nonomuraea sp. ATCC 39727 was maintained and cultivated to prepare genomic DNA according to [ 12].
Within the limitations of this in vitro study, all rotary nickel titanium instruments maintained the working length and prepared a well-shaped root canal without aberration.
Under the conditions of this study, all rotary NiTi instruments maintained the working length and prepared a well-shaped root canal.
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