Sentence examples for maintained and transfected from inspiring English sources

The cells were plated, maintained, and transfected on day 1 after plating using 5 μg of DNA and JetPrime transfection reagent (Autogen Bioclear) as described.

293T cells were maintained and transfected as described [18].

Other cells lines were maintained and transfected using synthetic siRNAs (Dharmacon) as described previously [21], [22].

N-WASP-deficient cells [60] were maintained and transfected as described.

HEK293 and COS-7 cells were maintained and transfected as described [18].

HeLa and Vero cells were maintained and transfected as described.

Because LAD2 cells expressed LPA5 receptors at levels comparable to that of primary hMCs and are easy to maintain and transfect, we focused our analysis on this cell line.

The transfection mixtures were removed at 6 h post transfection, and transfected cells were maintained for further processing.

MES23.5 cells, received with permission from Dr. Stanley Appel were maintained as described [22] and transfected using Lipofectamine 2000 according to the manufacturer's instructions.

We show that plasmids containing pbcen5 were both efficiently and stably maintained in transfected parasites during blood-stage multiplication, without drug pressure, demonstrating that pbcen5 can confer the function of a Plasmodium centromere.

HEK-293T and N1E-115 cells were maintained, transfected and infected as described previously (Niemann et al., 2009).