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Pretreatment with inhibitor of NADPH oxidase assembly, gp91 ds-tat significantly reduced the proportion of neurons undergoing apoptosis in the low magnesium model of seizure-like activity.
In keeping with this, we were able to abolish ROS production in the low magnesium model of seizure-like activity by NMDA receptor antagonists.
To confirm that increases in ROS production were not a peculiarity of the low magnesium model of seizure-like activity, we reproduced our findings using another in vitro epilepsy model.
In addition, 30 min preincubation with gp91 ds-tat (n=53), a specific peptide inhibitor of NADPH oxidase assembly, inhibited ROS production in the low magnesium model of seizure-like activity when compared with gp91 ds-tat scrambled (n=43; P<0.001; t-test; Figures 5e and f).
Despite studies suggesting that ROS of mitochondrial origin are the major source of ROS in epilepsy and specifically in the low magnesium model of epilepsy, we show here that ROS of mitochondrial origin are not the primary contributors to the neuronal free radical burden.
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Previous studies have suggested that increased ROS in the low magnesium model are of mitochondrial origin.
We therefore asked whether blocking sources of ROS generation might reduce cell death in the low magnesium model.
We therefore determined lipid peroxidation in the low magnesium culture model of seizure-like activity using C11-BODIPY, an indicator of lipid peroxidation.
To determine the mechanisms and sources of ROS generation, and their exact temporal relationship during the initial phase of network hyperexcitability, the well-established low magnesium culture model of seizure-like activity was used.
The various gating systems for a casting model of magnesium alloy were designed.
Adopting a flow stress model of magnesium alloy AZ31 considering the dynamic recrystallization (DRX), a 3D elastic plastic FE model of hot splitting spinning of magnesium alloy AZ31 is established based on the FEM software platform of ABAQUS/Explicit and a developed 3D-FE model of cold splitting spinning, and its reliability is validated.
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