After each peptide was serially diluted 100 μl of the lowest dilution inhibiting test strain were used for bacteriocin-like peptide (B), lipopeptide (L) and 50 μl of same dilutions were used to check the combined effect (B+L) on L. monocytogenes (I) and M. luteus (II).
Samples were serially diluted 1 2 to concentrations ranging from 6 µg/ml (undiluted) to 0.37 µg/ml (lowest dilution), and 2 µl were spotted onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare Life Sciences) and immunostained with specific anti-His mouse antibodies.
The dashed line represents the lowest dilution of tested sera.
Finally, an intellectualized longwall mining procedure was designed to guide the mining process with the lowest dilution and loss rates.
Standard deviation was noticeably highest at the lowest dilution of smoke (1 5) for both products tested.
It is expressed as the lowest dilution which inhibited growth, judged by the lack of turbidity in the tube.
The MIC value represents the lowest dilution of the compound at which no bacterial growth was detected.
Xylose consumption in the first-stage 1 L reactor was only 37% at the lowest dilution rate studied, 0.05 h−1.
The FI was calculated as the ratio of the highest enzyme dilution showing no star activity to the lowest dilution showing complete digestion (Wei et al., 2008).
Plates with colonies between 30 and 300 from the lowest dilution were counted and reported as colony-forming units per millilitre of milk (CFU/ml).
Overall, deposited mass detected within the chamber was notably less than for the 3R4F cigarette and ranged from 1.42 ±0.26 μg/cm2 (1,420 ng/cm2) at the lowest dilution of smoke (1 5), to 0.13 ±0.02 μg/cm2 (130 ng/cm2) at the highest dilution of smoke (1 100) during the 30 minutes smoke exposure (Table 1).
