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These rates of secondary dilution were much greater than model predictions.
The DMS was set to a secondary dilution of 1 60 to take α readings, as the smoke from the syringe at this stage was concentrated, but no secondary dilution for the β and γ readings was required.
For the secondary dilution holes, the pattern factor of Design A is better than that of Design B.
Rates of secondary dilution ranged from 2 to 3 orders of magnitude resulting in total dilutions of 105 106 within 3 h of discharge.
The present paper aims to study dilution holes including primary dilution holes and secondary dilution holes on the performance of a triple swirler combustor.
Similar(55)
After 3 times washing with PBS, cells were incubated with the Alexa 488-conjugated secondary antibody (dilution; 1∶1000, In Vitrogen) and DAPI (dilution; 1∶1000) for 1 hour at room temperature.
This was followed by adding horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (dilution 1 5000, Jackson, USA).
For nuclear staining, TO-PRO-3 (invitrogen) was added to the secondary antibody dilution.
PBS containing 0.2% Triton-X100 and 3% NDS (PBST) was used for primary and secondary antibodies dilution.
A FITC anti-rabbit secondary antibody (dilution 1∶200, Sigma) was then applied for 2 hours at RT in a humidified chamber.
Sections were then incubated with appropriate secondary antibodies (dilution 1∶1000) conjugated with Alexa Fluor 488, Alexa Fluor 555 or Alexa Fluor 568 (Invitrogen Ltd, Paisley, UK).
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