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Exact(4)
The G418R cell line that exhibited the highest level of complementation, designated CAN20, was subsequently used to generate and propagate udg-deletion recombinants of MVA (see below).
There was some variation in the level of complementation of the FA composition in the transgenic lines, which was likely due to positional effects of transgene insertion; this is a common phenomenon in the generation of transgenic plants.
By the same analysis we detected only a very limited amount of 3-hexaprenyl-4-hydroxyphenol (4HP6) in hu COQ6-isoa transformants (Fig. 1D), indicating that the human protein is not rate limiting for C5-hydroxylation and that the low level of complementation is probably due to an inefficient stabilization of the CoQ complex [5].
In contrast, due to the low viability of λ Dam15 on the SupD derivative, complementation by gpD::eGFP was observable as expression of the fusion increased with rising temperature and like the Sup− [pD::eGFP] strain, SupD[pD::eGFP] provided the greatest and a similar level of complementation for the Dam15 mutation at 39 40 °C.
Similar(56)
The slightly lower levels of complementation observed with B. parapertussis BteA correspond to its greater degree of divergence.
Furthermore, we found that the low level of protein complementation that occurred with co-expression of linker-luc1 and syn-luc2 was not significantly altered by overexpression of CHIP (Fig. 1E).
Conversely, the BRCA2 defect in CAPAN-1 could be fully responsible for the 100-fold defect in recombination if the level of BRCA2 complementation observed by Powell was incomplete.
Excel table containing the relative metabolite levels of the complementation lines and the PCA loadings.
Experimental studies with VSV have shown that relative fitness is MOI-dependent due to increased levels of strain complementation [ 13].
Unlike plant proteinase inhibitors, ovorubin is actively involved in the defense of the embryos not only by rendering them antinutritive, but also by providing them with a genetically encoded warning signal, comprising a new level of coordination and complementation of egg defenses.
On the basis of mapping data, the identification of lesions that disrupt the splicing or expression level of notch3 and complementation analysis, we conclude that both the st51 and zm mutations disrupt the function of notch3.
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