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The partial restoration in virulence observed with the C. jejuni ciaD complemented isolate is consistent with the results of complementation experiments performed by others using in vitro challenge studies [ 14, 43].
However, comparison to pyrG + complemented transformants showed that the increase was the results of complementation of the uridine deficiency alone.
Both lineages harbor eyeless fish populations, providing support for eye loss having evolved multiple times, a finding consistent with results of complementation studies (see above).
Similar results of complementation of the SUSY7/ ura3 yeast cells with tonoplast-localized HvSUT2 of barley [ 6], PtaSUT4 of populus [ 27] and expression-optimized OsSUT2 of rice [ 28] have been reported.
Results of complementation tests in the yeast mutant strain CP154-7A, defectine itsitwotwo sulfate transporters and thus unable to grow on media containing low concentrations of sulfate as the sole sulfur source [ 31], proved the capacity of BjSultr1 1 and BjSultr1 2a/b/c to transport sulfate ions across the plasma-membrane (Additional file 4).
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This result is consistent with the prediction that when co-infection is common, variants that have moderately inferior replicative ability are more likely to be maintained in the population as a result of complementation.
A 33-bp deletion in the sor1 mutant gene compromises the gravitropic response; the results of our complementation analysis indicate that the gravitropic response can be restored by a functional SOR1 gene.
β-galactosidase assays were done to verify the results of the complementation assays.
The results of the complementation analysis are summarized.
The data presented here are in accord with results of genetic complementation experiments, and support the hypothesis that different isoforms of dADA2b contribute to the functional variations of dSAGA multiprotein HAT complexes.
Furthermore, the results of the complementation tests with yet another overlapping deficiency at the same end (Df(1 BSC711 ) allowed us to subtract an additional 19.64 kb from the 210 kb encompassing Df(1 ED6906.
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