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KOH test was then employed to confirm the Gram's reaction (Quinn et al. 2004).

While the hyphae will darken when a solution of potassium hydroxide is applied (the KOH test), they remain otherwise unchanged.

Gram reaction was performed using the KOH test described by Gregersen [ 33].

A vaginal culture was evaluated for bacterial vaginosis and candidiasis using Nugent's scoring criteria, and the KOH test for candidiasis.

Specimen was sent for Gram stain, KOH test, aerobic bacteria, anaerobic bacteria, and fungus cultures, all of which were negative.

Patients with negative KOH test and patients who had no dystrophic nail changes were grouped together as OM negative patients.

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NaOH and KOH tests showed intermediate colour segregation i.e. light wine red colour compared with parents.

Studies on colour formation of aleurone layer revealed that IR 36 showed light yellow with NaOH and KOH tests, whereas, phenol and modified phenol tests recorded brown colour.

Similarly, IR 64 recorded light yellow colour with NaOH and KOH tests; while phenol and modified phenol tests were recorded dark brown colour as shown in Table 1.

Among them, 319 and 335 F2 progenies were wine red/dark wine red colour, whereas 165 and 149 F2 progenies were light yellow coloured with NaOH and KOH tests, respectively.

Further, the freshly harvested F1 seeds derived from the cross IR 64 × Acc. No. 2693 (Fig. 3) showed light wine red colour with NaOH and KOH tests, respectively.

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