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NaOH and KOH tests showed intermediate colour segregation i.e. light wine red colour compared with parents.
Studies on colour formation of aleurone layer revealed that IR 36 showed light yellow with NaOH and KOH tests, whereas, phenol and modified phenol tests recorded brown colour.
Similarly, IR 64 recorded light yellow colour with NaOH and KOH tests; while phenol and modified phenol tests were recorded dark brown colour as shown in Table 1.
Further, the freshly harvested F1 seeds derived from the cross IR 64 × Acc. No. 2693 (Fig. 3) showed light wine red colour with NaOH and KOH tests, respectively.
Among them, 319 and 335 F2 progenies were wine red/dark wine red colour, whereas 165 and 149 F2 progenies were light yellow coloured with NaOH and KOH tests, respectively.
The colour pattern with standard phenol/modified phenol, NaOH and KOH tests in F2 progenies of both the crosses showed 9 7 (complementary gene interaction) and 11 5 ratios (reciprocal dominance modification of recessive alleles), respectively.
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KOH test was then employed to confirm the Gram's reaction (Quinn et al. 2004).
Specimen was sent for Gram stain, KOH test, aerobic bacteria, anaerobic bacteria, and fungus cultures, all of which were negative.
Patients with negative KOH test and patients who had no dystrophic nail changes were grouped together as OM negative patients.
Isolated bacteria were identified in accordance with standard laboratory milk culture methodology, based on colony morphology, haemolysis, the catalase test, KOH test and Gram staining.
The diagnosis of OM was based on the presence of both positive clinical signs (dystrophic nail changes) and positive KOH test (OM positive group).
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