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Exact(56)
Fertilized eggs were collected on apple juice agar plates.
Larvae were grown on standard grape juice agar plates at low density at 25°C.
No haploid fruiting was observed when the samples were incubated alone on V8 juice agar.
Flies were then transferred to egg collection cages with grape juice agar plates and yeast for one hour.
Embryos were collected on standard apple juice agar plates and aged to stages 15 16 at 25 °C [48].
All strains were individually incubated on V8 juice agar as described above to differentiate haploid fruiting from mating.
We conducted crosses as described, and collected embryos on grape juice agar plates (Genesee) supplemented with live yeast.
Similar(4)
Highly virulent Phytophthora capsici strain SD33 has been tested in our laboratory and routinely cultured on 10% V8-juice agar medium at 25°C [ 51, 66, 67].
Embryos from dysgenic crosses were collected on freshly yeasted apple-juice agar plates and individual YFP-positive embryos were selected using the COPAS Select (Union Biometrica).
For growth assays, the P. capsici strain SD33 (WT), CK (only the selected gene expression), and the silenced transformations were subcultured twice on 10% V8-juice agar medium.
Embryos were collected on apple-juice agar plates and aged to 4 5.5 hr AEL (w 1118, wild type) or 6 7 hr AEL (His C mutant embryos).
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