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Re-suspended C. albicans cells were plated on the blood agar or Sabouraud dextrose broth agar plate for CFU counting.
A single colony of strain ATCC1803 from an anaerobic cooked meat broth agar plate was taken and cultured in 5 mL of cooked meat broth medium overnight.
Both of the strains were maintained on Luria broth agar slants.
Yeast nitrogen broth agar plates were inoculated with bacterial suspensions in mid-logarithmic phase by swabbing the agar surface.
In some cases, LB broth agar surfaces were treated with catalase (4000 or 10,000 units per plate) before stabbing of the test strain.
Particle incubation was then continued for further 20 h before cells were lysed, seeded on Lysogeny broth agar plates and incubated for 6 days.
Similar(11)
Transformed cells were spread onto Luria-Broth agar plates containing Ampicillin and incubated at 37°C overnight.
Briefly, 100-µl aliquots of 10-fold-diluted chemostat cultures were plated on L-broth agar plates in duplicate and incubated for overnight at 37 °C.
C6060-03) were isolated from commercial sources through plating on LB [Luria Bertani (broth)] agar (Biopolis Shared Facilities, A*STAR).
Pseudoalteromonas sp SANK 73390 and E. coli strains was grown on Marine broth/agar [1] at 23°C, Escherichia coli DH5α and S17-1 were grown on L Broth or L agar at 37°C [43] and Staphylococcus aureus NCTC 12493 and 16HLmupR1 were grown on Mueller Hinton II broth/agar at 37°C. Cloning vector pGEM-T (www.promega.com) and suicide plasmid pAKE604 [13] were described previously.
The BALF was serially diluted in phosphate-buffered saline and inoculated on Luria Broth agar plates.
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