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Appendix Tables 1 and 2 list the characteristics of the studied isolates by participating country, laboratory, laboratory code, clinical, veterinary or environmental origin, isolate characteristics (isolation year, isolation location, source, gender, age and risk factor), variety, serotype and the molecular type identified during this study.
The isolation year of each isolate was used to establish a temporal framework for constructing phylogenetic relationship among the isolates and estimating parameters to describe the evolutionary dynamics of the population (17 ).
Ten of the 120 isolates representing phylogenetic breadth based on isolation year and country (if applicable) were subjected to WGS for subsequent development of the evolutionary model and the SNP assay.
When we divided the strain collection based on isolation year and calculated the average number of SNPs separating the epidemic strains isolated in a given year from the reference strain, we noticed an increase in the number of polymorphisms differentiating the epidemic strains from the reference strain over time.
Further, sequences with the same animal host, isolation location, and isolation year were stratified by random sampling.
Strain name, sequence type based on MLST (ST), species, host, isolation location, isolation year, complex based on MLST [ 4], and references for each strain are summarized.
Similar(52)
The sampling time interval of these sequences is 21 years (isolation years ranging from 1986 to 2007).
All the genotype I clades consisted of Thai viruses with approximately similar isolation years (±7 years), suggesting that the same pool of DENV-1 strains circulated within Thailand [ 6].
From 1980 through 1985, a mean ± SD of 424.1 ± 281.3 specimens was submitted for isolation every year.
Because the isolates from this study were to a large extent unevenly distributed as to source of isolation versus year of isolation, analyzing resistance trends over time was not possible.
Syria has suffered international isolation for years, only to re-emerge as crises subside.
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