Exact(4)
The mobile phase consisted of an isocratic solution of 20 mM H2SO4.
The column was a Chiracel OD-R and the mobile phase was an isocratic solution of (5%, v/v) acetonitrile and (95%, v/v) 0.5 M NaClO4/HClO4 at pH 2.3.
After 100 fold dilution monosaccharides were determined using a Dionex HPLC system (Dionex Corp. Sunnyvale, Cal . USA fitted with a Carbo Pac PA-1 column 4-2500 mm), and a 25 μL sample loop with 20 mM NaOH isocratic solution (1 mL min-1) as the mobile phase.
Lipid classes were subsequently separated by HPLC using a silica column and a mobile phase consisting of a 20-min linear gradient from 100% solution A to 100% B (isopropanol-hexane-water, 8 6 1.5), followed by isocratic solution B for a further 25 min, with the column and sample collection system set at 20°C.
Similar(4)
These opposing interactions allow isocratic resolution [ 23].
Solvents A, B; composition: isocratic solvent mixtures with 45 to 55%% of MeOH.
The sample was injected onto a Higgins Haisil Semi-Preparative (5 μm, 250 mm × 10 mm) C-18 column and eluted with an isocratic program of 75% solution A and 25% solution B. The substrate purity was calculated to be greater than 99% on an LC MS/MS, although we measured a range of O labeling efficiencies from 57 to 78% in different labeling experiments.
The isocratic elution result showed that the concentration of liriodendrin reached maximum values when eluting with 2 BV of 40% v/v ethanol aqueous solutions.
Related(1)
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