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Ammonia and bromide were determined by Dionex-600 ion chromatography equipped with electrochemical detector (ECD-50), isocratic pump (IP25) using instrument manual.
This consisted of a Spectra-Physics 8810 isocratic pump.
Flow rate through the column was 0.23 ml/min and controlled by a Model LC1120 isocratic pump (GBC Scientific, Hampshire, IL).
Purified samples were placed into a 96-well plate holder or single vial holder of an Agilent 1200 Series HiP-ALS (High Performance Automated Liquid Sampler, G1367B) and sequentially injected by 0.8 ml/min flow from an Agilent 1200 Series Isocratic pump (G1310A).
An aliquot of 200 µl of OpuAC (0.2 0.4 mg/ml) was run at a flow rate of 0.5 ml/min on a Superdex 200 10/300gelgel filtration column (GE Healthcare) in 150 mM KPi pH 7.0 (+/−1 mM GB) using an Agilent 1200 series isocratic pump at room temperature.
Peptide solution (20 μL) was injected at 15 μL/min using an isocratic pump and trapped on a precolumn (Zorbax 300SB-C18, 5 mm × 0.3 mm, 5 μm).
Similar(34)
A lab scale microfluidic reactor with computer-controlled programmable isocratic pumps and online detectors was employed to syntheses CdSe nanocrystal and operational parameters for lab scale microfluidic reactor were optimized.
AF4 measurements of the PS-NP-exposed soil samples were performed in triplicate using an AF2000 MT system (Postnova Analytics, Landsberg, Germany) consisting of an autosampler (PN5300), two isocratic pumps (PN1130) a solvent degasser (PN7520), a cartridge oven (PN4020), and an analytical fractionation cartridge equipped with a 350 µm spacer and a 10 kDa regenerated cellulose membrane.
The setup used for the online assay included two LC-20 AD and two LC-10 AD isocratic pumps, a SIL-20 AC autosampler, one CTO-20 AC and one CTO-10 AC column oven, an RF-10AXL fluorescence detector, a CBM-20A controller, and an ion trap time-of-flight (IT-TOF) mass spectrometer equipped with an ESI source, all products of Shimadzu ('s Hertogenbosch, the Netherlands).
End-product analyses were performed as previously described (Gungormusler et al. [2011b]) using high performance liquid chromatography (Waters Breeze™ HPLC System, USA) with a Phenomenex Rezex RHM Monosaccharide (H+) 300 x 7.8 mm ion exchange column, using a Waters 2414 Refractive Index Detector, a Waters 1515 Isocratic HPLC Pump, and a Waters 2707 Autosampler.
It was washed for 5 min with the equilibration solution at a flow rate of 25 µL/min by using an isocratic loading pump operated through an auto sampler.
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