This leads to a significant deterioration in overall isocratic separation performance.
Isocratic separation method was utilized with a flow rate of 0.7 ml/min and column temperature set to 25 °C.
The isocratic separation method involved an octadecylsilane (ODS) column and an acetonitrile toluene (1 1) mobile phase.
The theoretical results are verified with specially designed experiments on isocratic separation of linear polystyrenes on a column packed with non-porous particles at various solvent compositions.
Although column retention appears dependent upon residual charge on the imine function, successful isocratic separation can be achieved by secondary hydrophobic interactions.
And the agreement between the experimental data and the calculated values was relatively good using isocratic separation for eight isoflavones, but the retention time is very long.
This technique involves the use of a pre-column derivatization with 2,3-naphthalenedialdehyde (NDA), an isocratic separation in presence of β-cyclodextrine and a fluorimetric detection.
But for isocratic separation of the low-molecular-weight, organic compound is relatively poor in polymeric monoliths; it is because of an absence of mesopore, a presence of micropore, and a structural inhomogeneity (causing flow dispersion) (Guiochon, 2007; Svec, 2010).
