Isocratic method was found to be better for the assay.
Total mannan and glucan were determined by HPLC using an isocratic method and a Refractive Index (RI) Detector.
An isocratic method was developed to analyze purified samples that mainly contained saponins 3 and 4, the most active saponins.
Crotonic acid was determined using a high-performance liquid chromatography system (Thermo-Fischer Scientific, USA) with a Nucleosil C8 column (Macherey Nagel, Germany) using an isocratic method.
The objective of the ion chromatographic method is to develop the novel isocratic method for assay determination in Aza sugars having polyhydroxyl compounds such as 1-deoxynojirimycin, miglitol, castanospermin, and celgosivir.
The isocratic method was used with pH adjusted to 2.5, using orthophosphoric acid and a solvent mixture of acetonitrile and deionized water (60 40% v/v) at a flow rate of 1 mL min−1 at room temperature.
Solutes: MEG: 1; amide: 2; lauric acid: 3; amide-ester: 4. Isocratic method: mobile phase: methanol: water: TFA 75250.3.3 v/v ; flow rate 1 ml min−1 and UV wavelength set at 210 nm.
A SphereClone SAX 5 μm 250 × 4.6 mm column (Phenomenex, Torrance, CA, USA) was used for RP-HPLC at a flow rate of 0.8 ml min−1 with UV detection at 295 nm, using an isocratic method with 20 mmol l−1 potassium phosphate (pH 7.2) as the mobile phase.
